人乙醇脫氫酶(ADH)ELISA Kit說(shuō)明書(shū)

Elisa實(shí)驗(yàn)常用方法： 
雙抗體夾心法測(cè)定抗原 

將抗原免疫*種動(dòng)物獲得的抗體吸附于固相表面;

加抗原，形成抗原-抗體復(fù)合物; 

加抗原免疫第二種動(dòng)物獲得的抗體，形成抗體抗原抗體復(fù)合物;

加酶標(biāo)抗抗體(第二種動(dòng)物抗體的抗體); 

加底物。底物的降解量＝抗原量。

人乙醇脫氫酶(ADH)ELISA Kit說(shuō)明書(shū)

Elisa實(shí)驗(yàn)原理：酶結(jié)合物與相應(yīng)抗原或抗體結(jié)合后，可根據(jù)加入底物的顏色反應(yīng)來(lái)判定是否有免疫反應(yīng)的存在，而且顏色反應(yīng)的深淺是與標(biāo)本中相應(yīng)抗原或抗體的量成正比例的，所以，可以按底物顯色的程度顯示試驗(yàn)結(jié)果。 

人乙醇脫氫酶(ADH)ELISA Kit說(shuō)明書(shū)

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