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上海研盟生物科技有限公司Anti-CACNB3抗體*，主要應(yīng)用于WB、IHC、IF、ELISA、流式細胞術(shù)等實驗中。說明書隨貨發(fā)送，您也可以直接我司在線客服索取?？头?/span>

英文名稱：Anti-CACNB3 antibody

中文名稱：L型電壓依賴型鈣通道β3(L-type Ca++ CPβ3)抗體

L型電壓依賴型鈣通道β3(L-type Ca++ CPβ3)抗體保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

★研盟生物★L型電壓依賴型鈣通道β3(L-type Ca++ CPβ3)抗體,Anti-CACNB3抗體

免疫組化操作步驟

（一）、儀器設(shè)備

1、 18cm不銹鋼高壓鍋或電爐或用微波爐

2、 水浴鍋

（二）、試 劑

1、 PBS緩沖液（pH7.2―7.4）

2、 0.01mol/L檸檬酸鈉緩沖液（CB,pH6.0,1000ml）

3、 3%甲醇―H2O2溶液：用30% H2O2 和80%甲醇溶液配制

4、 風裱劑：

a. 甘油和0.5mmol/L碳酸鹽緩沖液（pH9.0–9.5）等量混合

b. 油和TBS（PBS）配制

5、 TBS/PBS pH9.0–9.5，適用于熒光纖維鏡標本；pH7.0-7.4適合光學纖維標本

（三）、操作流程

1、 脫蠟和水化：脫蠟前應(yīng)將組織芯片在室溫中放置60分鐘或60℃恒溫箱中烘烤20分鐘

a. 組織芯片置于二甲苯中浸泡10分鐘，更換二甲苯后在浸泡10分鐘

b. 無水乙醇中浸泡五分鐘

c. 95%乙醇中浸泡五分鐘

d. 75%乙醇中浸泡五分鐘

2、 抗原修復(fù)：用于福爾馬林固定的石蠟包埋組織芯片

L型電壓依賴型鈣通道β3(L-type Ca++ CPβ3)抗體,Anti-CACNB3抗體溶解方法:

方法1：我們的抗體（凍干粉）已經(jīng)包含了0.01M PBS、1% BSA和0.1% 防腐劑（疊氮鈉或*），您只需要加入滅菌的雙蒸水就行了?？贵w溶解后，置于-20℃保存，分裝后使用，避免反復(fù)凍融，可保證至少1年有效；

方法2：也可以只加入一半體積的雙蒸水，再用一半體積的甘油補足，置于-20℃保存，這樣抗體不會凍，有利于抗體的穩(wěn)定。

后續(xù)試驗時，再使用對應(yīng)的緩沖液稀釋成工作液，即用即配。

我們的抗體（凍干粉），在常溫放置下，至少一個月有效；若在-20℃下保存（*），至少三年有效。

Antibodies are prepared with 0.01M PBS, pH 7.4 with 1% BSA and 0.1% Sodium Azide, prior to lyophilization. Currently, we offer both lyophilized and reconstituted antibodies, both resulting in final concentration of 1mg/mL. We recommend two ways to reconstitute antibodies in lyophilized form:

1) Reconstituting by directly adding 100uL of sterile, distilled water.

2) Alternatively, if glycerol does not affect the experiment, add 50uL sterile distilled water, followed by adding 50uL of glycerol.

L型電壓依賴型鈣通道β3(L-type Ca++ CPβ3)抗體,Anti-CACNB3抗體簡單介紹：

產(chǎn)品別名：CAB3; CACB3_HUMAN; CACNLB3; Calcium Channel Voltage Dependent Beta 3 Subunit; Calcium channel voltage-dependent subunit beta 3; FLJ58949; Voltage-dependent L-type calcium channel subunit beta-3.  

抗體來源：Rabbit or Mouse

保質(zhì)期:1年

克隆類型：Polyclonal or monoclonal

性    狀：Lyophilized or Liquid

濃    度：1mg/1ml

背景介紹：Voltage-dependent calcium channels are essential for the release of neurotransmitters. L-type (long lasting current) voltage-dependent calcium channels are composed of four subunits: an Alpha1 subunit, a Beta subunit, a Beta subunit and an Alpha2 Gamma subunit. The Beta subunit is encoded by four genes, designated Beta1-Beta4, all of which contribute to the diversity of calcium currents and are involved in membrane trafficking of the Beta subunit. L-type Ca++ CP Beta3, also known as CACNB3 (Calcium channel voltage-dependent subunit beta 3), CACNLB3 or CAB3, is a 484 amino acid protein that contains one SH3 domain and is expressed in ovary, brain and smooth muscle. Functioning as one of the four components of the Beta subunit, L-type Ca++ CP Beta 3 increases the peak calcium current in voltage-dependent calcium channels, thereby shifting the voltage dependencies of activation and inactivation and controlling G protein inhibition and Beta membrane targeting. Two isoforms of L-type Ca++ CP Beta3 exist due to alternative splicing events.

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