人上皮細(xì)胞粘附分子(Ep-CAM/CD362)ELISA Kit
ELISA方法的基本原理是酶分子與抗體或抗抗體分子共價(jià)結(jié)合,此種結(jié)合不會(huì)改變抗體的免疫學(xué)特性,也不影響酶的生物學(xué)活性。此種酶標(biāo)記抗體可與吸附在固相載體上的抗原或抗體發(fā)生特異性結(jié)合。滴加底物溶液后,底物可在酶作用下使其所含的供氫體由無(wú)色的還原型變成有色的氧化型,出現(xiàn)顏色反應(yīng)。因此,可通過(guò)底物的顏色反應(yīng)來(lái)判定有無(wú)相應(yīng)的免疫反應(yīng),顏色反應(yīng)的深淺與標(biāo)本中相應(yīng)抗體或抗原的量呈正比。此種顯色反應(yīng)可通過(guò)ELISA檢測(cè)儀進(jìn)行定量測(cè)定,這樣就將酶化學(xué)反應(yīng)的敏感性和抗原抗體反應(yīng)的特異性結(jié)合起來(lái),使ELISA方法成為一種既特異又敏感的檢測(cè)方法。
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上海研拓生物科技有限公司專(zhuān)業(yè)經(jīng)銷(xiāo)眾多生命科學(xué)領(lǐng)域的ELISA試劑盒,生物試劑,抗體,培養(yǎng)基,血清。為中國(guó)科研試劑供應(yīng)商之一,質(zhì)量被全國(guó)各大院??蒲袡C(jī)構(gòu)認(rèn)可,并為Elisa試劑盒*供應(yīng)商,作為戰(zhàn)略合作伙伴。選購(gòu),期待與您合作!